Fig. 6From: SNP55, a new functional polymorphism of MDM2-P2 promoter, contributes to allele-specific expression of MDM2 in endometrial cancersThe effect of SNP55 on MDM2-P2 promoter activity. a The pGL4 luciferase reporter plasmids containing either SNP55T or SNP55C were co-transfected with pcDNA-LacZ (control) and pcDNA-Sp1 (N-terminus myc-tagged). b Western blot analysis confirming a gradient increase of nuclear factor kappa-B (NFκB) p50. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is shown as an internal control. c The amount of pcDNA- nuclear factor kappa-B (NFκB) p50 (0–0.8 μg) was gradually increased and co-transfected with the SNP55C reporter plasmid. d The amount of pcDNA- nuclear factor kappa-B (NFκB) p50 (0–0.8 μg) was gradually increased and co-transfected with the SNP55T reporter plasmidBack to article page