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Table 5 Quintuplex (n=5) pyro-signals (n=10): results of AdvISER-MH-PYRO after adjustement of primer concentrations

From: Multiplex pyrosequencing assay using AdvISER-MH-PYRO algorithm: a case for rapid and cost-effective genotyping analysis of prostate cancer risk-associated SNPs

Patient

rs10896449

rs1859962

rs4242382

rs4430796

rs6983267

R

 

Genotype

Contriution a (%)

Genotype

Contribution a (%)

Genotype

Contribution a (%)

Genotype

Contribution a (%)

Genotype

Contribution a (%)

 

1

G/G

5.51 (19.4)

G/G

8.79 (30.9)

G/G

5.75 (20.2)

A/A

3.24 (11.4)

G/T

5.13 (18.1)

0.998

2

A/G

5.82 (23.2)

T/T

4.05 (16.1)

G/G

6.91 (27.5)

A/A

2.33 (9.3)

G/T

6.00 (23.9)

0.998

3

A/G

5.67 (21.0)

T/T

3.96 (14.7)

G/G

7.63 (28.3)

G/G

3.31 (12.3)

G/T

6.40 (23.7)

0.999

4

A/G

5.07 (20.6)

T/G

3.50 (14.2)

G/G

6.44 (26.1)

A/A

3.30 (13.4)

G/G

6.36 (25.8)

0.996

5

G/G

5.55 (19.3)

T/T

3.52 (12.2)

G/G

7.74 (26.9)

G/A

3.14 (10.9)

T/T

8.86 (30.8)

0.999

6

A/G

7.28 (22.4)

T/T

4.91 (15.1)

G/G

7.47 (23.0)

G/A

5.21 (16.0)

T/T

7.64 (23.5)

0.998

7

A/G

6.35 (21.1)

T/G

4.28 (14.2)

G/G

8.22 (27.3)

G/A

4.28 (14.2)

G/G

7.00 (23.2)

0.996

8

A/A

0.94 (10.7)

T/T

1.47 (16.7)

G/G

2.82 (32.1)

A/A

1.57 (17.9)

G/T

1.99 (22.6)

0.998

9

G/G

4.73 (18.6)

T/T

4.43 (17.4)

A/G

6.80 (26.8)

G/A

2.17 (8.5)

T/T

7.27 (28.6)

0.997

10

A/G

4.23 (14.5)

G/G

3.07 (14.5)

G/G

6.15 (29.1)

G/A

2.50 (11.8)

G/T

5.16 (24.4)

0.999

Average

 

(19.3)

 

(16.6)

 

(26.7)

 

(12.6)

 

(24.4)

 
  1. aFor each patient, the genotype and its absolute and relative contributions to the global signal are computed by AdvISER-MH-PYRO for each SNP, R: confidence index traducing the correlation between the observed multiplex pyro-signal and the sparse regression model constructed by AdvISER-MH-PYRO