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Table 4 Quadruplex (n=4) pyro-signals (n=10): results of AdvISER-MH-PYRO after adjustement of primer concentrations

From: Multiplex pyrosequencing assay using AdvISER-MH-PYRO algorithm: a case for rapid and cost-effective genotyping analysis of prostate cancer risk-associated SNPs

Patient

rs1016343

rs10993994

rs16901979

rs5945619

R a

 

Genotype

Contribution a (%)

Genotype

Contribution a (%)

Genotype

Contribution a (%)

Genototype

Contribution a (%)

 

1

C/C

4.36 (18.6)

T/T

2.87 (12.2)

C/C

11.35 (48.4)

T

4.85 (20.7)

0.999

2

C/C

5.52 (20.5)

C/C

7.05 (26.2)

C/C

10.71 (39.8)

T

3.66 (13.6)

0.998

3

C/C

4.58 (19.6)

C/C

3.66 (15.6)

C/C

11.09 (47.4)

C

4.09 (17.5)

0.999

4

C/C

2.85 (16.4)

C/C

2.23 (12.8)

C/C

9.78 (56.1)

T

2.57 (14.7)

0.999

5

C/C

4.97 (18.8)

C/T

5.34 (20.2)

C/C

10.63 (40.3)

T

5.44 (20.6)

0.999

6

T/T

3.92 (16.3)

C/T

4.93 (20.5)

C/C

10.2 (42.5)

T

4.95 (20.6)

>0.999

7

C/C

3.98 (17.9)

C/T

3.87 (17.4)

C/C

10.23 (45.9)

T

4.2 (18.9)

0.997

8

C/T

4.87 (18.3)

C/T

5.19 (19.5)

A/A

12.41 (46.6)

T

4.18 (15.7)

0.999

9

C/C

3.05 (18.4)

C/C

1.82 (11.0)

C/C

9.18 (55.3)

C

2.56 (15.4)

0.999

10

C/C

2.72 (16.2)

C/C

2.35 (14.0)

C/C

9.29 (55.2)

T

2.46 (14.6)

>0.999

Average

 

18.1

 

16.9

 

47.7

 

17.2

 
  1. aFor each patient, the genotype and its absolute and relative contributions to the global signal are computed by AdvISER-MH-PYRO for each SNP, R: confidence index traducing the correlation between the observed multiplex pyro-signal and the sparse regression model constructed by AdvISER-MH-PYRO