SNP | Quadruplex (n=4) | Multiplex PCR | Multiplex PYRO | |||
---|---|---|---|---|---|---|
Quintuplex (n=5) | Conc.(μM) | |||||
Primer | Initial a | Adjusted a | Primer | Conc.(μM) | ||
rs1016343 | 4 | F: 5’-Biot-TCAGGGCAATTACGGAATAACA-3’ | 0.1 | 0.1 | 5’-TGAAGCTGTGAGTAATCA-3’ | 0.4 |
R: 5’-AATCTAAAGAATGGGGGTCAGAG-3’ | 0.1 | 0.1 | ||||
rs10993994 | 4 | F: 5’-CTCTCCTCCTCTGCTCTTTTAGGT-3’ | 0.1 | 0.1 | 5’-TTGTTATCATTCCCAA-3’ | 0.4 |
R: 5’- Biot- AGGCAAAGCTGCATCAAACT-3’ | 0.1 | 0.1 | ||||
rs16901979 | 4 | F: 5’-GTGGGGTCTTTGTTGTGGA-3’ | 0.1 | 0.1 | 5’-AATGATTTAGCATTACTTAT-3’ | 0.4 |
R: 5’-Biot-TTATGTTCAGAGCGGTTGAATG-3’ | 0.1 | 0.1 | ||||
rs5945619 | 4 | F: 5’-Biot-CAGGAAGGGGAACATCACACT-3’ | 0.1 | 0.05 | 5’-CTTGCGGGAGTCTCA-3’ | 0.4 |
R: 5’- ACGGCTACTATGAGATGAGGAAAC-3’ | 0.1 | 0.05 | ||||
rs10896449 | 5 | F: 5’-Biot-GGGCCACAGGGAACAA-3’ | 0.1 | 0.15 | 5’-TGACATTCCCCTTCTTA-3’ | 0.4 |
R: 5’-GCCTTTTCAATGAACCCACA-3’ | 0.1 | 0.15 | ||||
rs1859962 | 5 | F: 5’-AATAAGAGGCTGCAGACTTTTCC-3’ | 0.1 | 0.05 | 5’-AAATCCCTGCCCGTG-3’ | 0.4 |
R: 5’-Biot-TAGGCATTCCAAAGATGAAGACTC-3’ | 0.1 | 0.05 | ||||
rs4242382 | 5 | F: 5’-AAAAGAGGTAACCCAGGGAACA-3’ | 0.1 | 0.075 | 5’-TTGTCCCTCTAGTTATCTTC-3’ | 0.4 |
R: 5’-Biot-GCATAGAGGGACGCTGTCAA-3’ | 0.1 | 0.075 | ||||
rs4430796 | 5 | F: 5’-ACGTCCCTTCCTCAGCATCTT-3’ | 0.1 | 0.3 | 5’-GGCAGCACAGACTGGA-3’ | 0.4 |
R: 5’-Biot-TGTTCCTGACATGAAGCAACTCT-3’ | 0.1 | 0.3 | ||||
rs6983267 | 5 | F: 5’-Biot-TCTTCCTATCTCAGCTCCCTATCC-3’ | 0.1 | 0.1 | 5’-AATTCTTTGTACTTTTCTCA-3’ | 0.4 |
R: 5’-GTTGGCTGGCACTGTCTGT-3’ | 0.1 | 0.1 |