Fig. 1

Anti-miR-206 partially antagonizes the effect of p19 on G1/S and G2 phases. a Fold change is the value used to measure overexpression of pRK5-p19 as compared first to pRK5 empty vector and then to overexpression of pRK5-p19mut for miRNA microarrays. (*) indicates the value obtained with pRK5-p19mut. These miRNAs varies upon overexpression of 19 and not by p19mut overexpression. Additional gene information is available in Additional file 1 (P < 0.01). RT: miR-206 activation was confirmed by Taqman RT-PCR with a specific Taqman assay for mature miR-206 (Applied Biosystems). b HeLa cells were first transfected with the pSuper-GFP vector containing the sequence of anti-miR-206 and incubated for 2 days under cell-culture conditions. Cells were then re-transfected with GFP-p19 vector and analyzed by FACS (fluorescent-activated cell sorting) the following day. A representative flow cytometry histogram with GFP-p19, pSuper-GFP anti-miR-206, GFP-p19 + pSuper-GFP anti-miR-206 or GFP only (negative control) is shown in the upper portion, whereas G1, S, and G2 percentages are given below (*P < 0.001 as compared G1 and G2 to GFP-19 versus GFP-p19 + anti-miR-206). HeLa cells line were used in all the experiments stated above