Table 1 Primer sequences and length of PCR/RFLP products
Polymorphism | Primer sequence* | Length of PCR or RFLP product, bp | |
|---|---|---|---|
Wild type allele | Polymorphic allele | ||
GSTM1 null polymorphism | F: 5′-GAACTCCCTGAAAAGCTAAAGC-3′ | 215 | No product |
R: 5′-GTTGGGCTCAAATATACGGTGG-3′ | |||
GSTT1 null polymorphism | F: 5′-TTCCTTACTGGTCCTCACATCTC-3′ | 480 | No product |
R: 5′-TCACCGGATCATGGCCAGCA-3′ | |||
ALB (positive control) | F: 5′-GCCCTCTGCTAACAAGTCCTA′-3 | 350 | - |
R: 5′-GCCCTAAAAAGAAAATCGCCAATC′-3 | |||
GSTP1 A313G | F: 5′-CCAGTGACTGTGTGTTGATC-3′ | 189 | 148; 41 |
R: 5′-CAACCCTGGTGCAGATGCTC-3′ | |||
UGT1A1 A(TA)nTAA | F: 5′-GTCACGTGACACAGTCAAAC-3′ | 98 | 100 |
R: 5′-TTTGCTCCTGCCAGAGGTT-3′ | |||
SULT1A1 G638A | F: 5′-GTTGGCTCTGCAGGGTTTCTAGGA-3′ | 166; 167 | 333 |
R: 5′-CCCAAACCCCCTGCTGGCCAGCACCC-3′ | |||