Figure 1From: Investigation of prognostic value of polymorphisms within estrogen metabolizing genes in Lithuanian breast cancer patientsThe electrophoresis results of PCR products and restriction endonuclease digestion. A. PCR products of GSTM1 and GSTT1. Lane M: DNA Ladder; Lane 1: Negative control (no DNA); Lanes 2, 5, 9, 12: GSTM1 null, GSTT1 present; Lanes 3, 4, 6, 7, 10, 11: GSTM1 present, GSTT1 present; Lane 8: GSTT1 null, GSTM1 present. B. Digestion products of GSTP1. Lane M: DNA Ladder; Lanes 1 and 4: AA; Lane 2: Heterozygous positive control for digestion; Lane 3: AG; Lane 5: GG; Lane 6: Negative control. C. Digestion products of SULT1A1. Lane M: DNA Ladder; Lane 1: Negative control; Lane 2: AG; Lane 3: GG; Lanes 4 and 5: AA; Lane 6: Positive control for digestion. D. PCR products of UGT1A1. Lane M: DNA Ladder; Lane 1: Negative control; Lane 2: 6/6; Lane 3: 6/7; Lanes 4 and 5: 7/7. The DNA ladders are used for approximate sizing of DNA fragments. The discrepancy between the size of digestion products and DNA ladder fragments in panel B may be due to the different fragment migration in gel resulting from different GC/AT content. The 41 bp fragment is extremely faint and masked by the tracking dye in panel B.Back to article page