Skip to main content
Figure 1 | BMC Medical Genetics

Figure 1

From: Investigation of prognostic value of polymorphisms within estrogen metabolizing genes in Lithuanian breast cancer patients

Figure 1

The electrophoresis results of PCR products and restriction endonuclease digestion. A. PCR products of GSTM1 and GSTT1. Lane M: DNA Ladder; Lane 1: Negative control (no DNA); Lanes 2, 5, 9, 12: GSTM1 null, GSTT1 present; Lanes 3, 4, 6, 7, 10, 11: GSTM1 present, GSTT1 present; Lane 8: GSTT1 null, GSTM1 present. B. Digestion products of GSTP1. Lane M: DNA Ladder; Lanes 1 and 4: AA; Lane 2: Heterozygous positive control for digestion; Lane 3: AG; Lane 5: GG; Lane 6: Negative control. C. Digestion products of SULT1A1. Lane M: DNA Ladder; Lane 1: Negative control; Lane 2: AG; Lane 3: GG; Lanes 4 and 5: AA; Lane 6: Positive control for digestion. D. PCR products of UGT1A1. Lane M: DNA Ladder; Lane 1: Negative control; Lane 2: 6/6; Lane 3: 6/7; Lanes 4 and 5: 7/7. The DNA ladders are used for approximate sizing of DNA fragments. The discrepancy between the size of digestion products and DNA ladder fragments in panel B may be due to the different fragment migration in gel resulting from different GC/AT content. The 41 bp fragment is extremely faint and masked by the tracking dye in panel B.

Back to article page

BMC Medical Genetics

ISSN: 1471-2350

Contact us