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Figure 3 | BMC Medical Genetics

Figure 3

From: Alternative splicing in osteoclasts and Paget’s disease of bone

Figure 3

Western blot analysis of PIDD , TBC1D25 , RHOT1 , USP4 and LGALS8 encoded proteins in pagetic or control OCs. The protein expression of the candidate genes was analyzed in OC cultures derived from PDB or HD without any p62 mutations. A- Western blot analyses of each encoded protein were performed using antibodies directed against PIDD, TBC1D25, RHOT1, USP4 and LGALS8 proteins or actin. B- Optical densities for bands corresponding to each protein were corrected with the optical density obtained for bands corresponding to actin, and computed in graphical representations (n = 4 to 6 in each group, in 2 independant experiments). Analyses are reported as mean ratio ± SD (*p ≤ 0.05, ** p ≤ 0.01).

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