Figure 2
From: Functional analysis of the novel TBX5c.1333delC mutation resulting in an extended TBX5 protein
Intracellular localization of wild type and mutant TBX5. (A) COS-7 cells were transfected with TBX5 fused to the sequence encoding the green fluorescent protein (GFP). Wild type TBX5 appeared in dot-like structures in the nuclei (blue, stained with DAPI) of the analyzed cells. (B) The mutant TBX5 displays a distinct and homogenous arrangement in the nucleus, the distribution pattern does not differ compared to the wild type TBX5. (C) The empty pEGFP-N3-vector was transfected as a negative control and resulted in a diffuse nuclear staining.