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Table 2 Primers and PCR programs for ADAM33 PCR-RFLP genotyping

From: Association of ADAM33 gene polymorphisms with adult allergic asthma and rhinitis in a Chinese Han population

Reference SNP ID

Gene/SNP primer

Sequence

PCR program

rs2787094

ADAM33 V4C/G F

5'-ACACACAGAATGGGGGAGAG-3'

94°C 5 min; 35 cycles, 94°C 30 s,

 

ADAM33 V4C/G R

5'-CCAGAAGCAAAGGTCACACA-3'

53°C 30 s, 72°C 30 s; 72°C 5 min

rs2280089

ADAM33T+1A/G F

5'-CTGAGCCCAGAAACCTGATT-3'

94°C 5 min; 35 cycles, 94°C 30 s,

 

ADAM33T+1A/G R

5'-AGAAGGGAAGGGCTCATGC-3'

54°C 30 s, 72°C 30 s; 72°C 5 min

rs2280090

ADAM33 T2A/G F

5'-TTCTCAGGGTCTGGGAGAAA-3'

94°C 5 min; 35 cycles, 94°C 30 s,

 

ADAM33 T2A/G R

5'-GCCAACCTCCTGGACTCTTA-3'

52°C 30 s, 72°C 30 s; 72°C 5 min

rs2280091

ADAM33 T1A/G F

5'-ACTCAAGGTGACTGGGTGCT-3'

94°C 5 min; 35 cycles, 94°C 30 s,

 

ADAM33 T1A/G R

5'-GAGGGCATGAGGCTCACTTG-3'

54°C 30 s, 72°C 30 s; 72°C 5 min

rs3918396

ADAM33 S1A/G F

5'-TGTGCAGGCTGAAAGTATGC-3'

94°C 5 min; 35 cycles, 94°C 30 s,

 

ADAM33 S1A/G R

5'-AGAGCTCTGAGGAGGGGAAC-3'

50°C 30 s, 72°C 30 s; 72°C 5 min

rs612709

ADAM33 Q-1A/G F

5'-GGATTCAAACGGCAAGGAG-3'

94°C 5 min; 35 cycles, 94°C 30 s,

 

ADAM33 Q-1A/G R

5'-GTTCACCTAGATGGCCAGGA-3'

60°C 30 s, 72°C 30 s; 72°C 5 min