Table 1 Primer sequences and annealing temperatures
From: Autosomal dominant hereditary spastic paraplegia: Novel mutations in the REEP1 gene (SPG31)
Amplified segment | Primer | Annealing temperature |
|---|---|---|
SPG31 Ex 1 | F1: ACCAGCTCACCGCCCAATC R1: GTATTAATAGCCCGAGCCACTGG | 56°C/30 cycles |
SPG31 Ex 2 | F2: AAGATAGAGGTGCCCAAGGATG R2: TGAGAACTGTGATGAAGGAACTCC | 58°C/28 cycles |
SPG31 Ex 3 | F3: GGAAGGATAGGGAGAAGGCTAC R3: CCTGAGGTGAGGTTTGAGGC | 58°C/28 cycles |
SPG31 Ex 4 | F4: TTCAAAAGGGCAGTGTTGCTG R4: ATGACTTGCTGGAGGCAAATGA | 58°C/28 cycles |
SPG31 Ex 5 | F5: GCCAAGCCCAATGCTCAGAG R5: GGTCCTTAGCCTGTTCTGTGTGG | 60°C/28 cycles |
SPG31 Ex 6 | F6: AGCTCCCTCTTGGCCTTTGTC R6: GGACTGGGCCTCTCTCAATGA | 58°C/28 cycles |
SPG31 Ex 7 | F7: GAGATTCAGGGAGACCCCAGC R7: GGAGAGAGAAAAGGCCATGTTTG | 60°C/28 cycles |