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Table 3 Primer sequences used for methylation specific PCR

From: Methylation of class II transactivator gene promoter IV is not associated with susceptibility to Multiple Sclerosis

Primers

Sequence

Product Size

Methylated Forward

TGTTTGGTTGTTTTATAGTTTGGTTC

60 bp

Methylated Reverse

CTACTAATAACCTCTCCCTCCCG

 

Unmethylated Forward

TTGGTTGTTTTATAGTTTGGTTTGA

157 bp

Unmethylated Reverse

CTACTAATAACCTCTCCCTCCCAC

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