From: Genetic and biochemical studies in Argentinean patients with variegate porphyria
Amplified region (exon) | Amplified Region (intron) | MW (pb) | Sequence (5' to 3') | Annealing (°C) | Mg+2 (mM) | |
---|---|---|---|---|---|---|
I | 2, 3, 4 | 2, 3 | 758 | F: GCTTCTGGAGCGCAGGTTGTCC R: AAGGCATATGAGGATGAGGGCA | 60 | 1.6 |
II | 5, 6 | 5 | 680 | F: AGGTATGTCAGGAGCTTCCCCC R: CCCTCACTTTGGCAGTACTTAA | 60 | 1.6 |
III | 7, 8 | 7 | 852 | F: TGCTGGGATTACAGGTGT R: AGCTTTTGCTTCTCACTGGTAGG | 62 | 2.5 |
IV | 9 | ----------- | 321 | F: GATTACAGGTGTGAGCCACCA R: CCTACCAGTGAGAAGCAAAAGCT | 60 | 2 |
V | 10, 11,12, 13 | 10,11,12 13 | 859 | F: GCCCTTTCCTTCTGACGCATG R: GCCAGACCAAGCCAAGCCAAGC | 62 | 2.5 |
VI | 1 | 1 | 440 | F: CCAAGTCCCGCCAATCCAGAT R: GGACAACCTGCGCTCCAGAAGC | 60 | 5 |
VII | Promotor | ----------- | 705 | F: AGGTGATAGAGAACTGGCCCAA R: CAGCCTTTTCGGTCTCTCCTA | 63 | 3 |
VIII | ------------ | 4 | 786 | F: TCTGAGCTTGGCTTGGATTC R: CTCTGCAGCTGGTTTAGG | 60 | 2 |
IX | ------------ | 6 | 528 | F: GCTTTCCCAGTCTCTTCC R: AAGGCCTGGCGAATGAGT | 60 | 2 |
X | ------------ | 8 | 313 | F: ATTCTCATTTTCTGGGTCTCTC R: TCAGCAGGGAGCAGCTCACTG | 60 | 2 |
XI | ------------ | 9 | 535 | F: CTGAGTGCCATCACTGCA R: GCTCAGGGAAAGCAACTG | 60 | 2 |
XII | Gen | All | 5,500 | F: AGAGAACTGGCCCAAAATTGGAGT R: CAGACCAAGCCAAGCCAAGCAATT | 60 | 2 |
4–9 | From nt 226 (exon 4) to nt 893 (exon 9). | 668 | F: TCTGAGCTTGGCTTGGATTC R: GTCACTCGACGAGGGACGACT | 60 | 3 | |
6–9 | From nt 478 (exon 6) to nt 893 (exon 9). | 416 | F: TCTCTAGCCATGGACAGTCT R: GTCACTCGACGAGGGACGACT | 60 | 6 |