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Table 2 Redesigned primer pairs for HRM

From: Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM

Exon

Oligo (5' – 3')

product size

HA – 1-1F

CCACTGATAAAAAGGAAGCA

226

HA – 1-1R

GTGGAGAGCTCTATTTGCAT

 

HA – 1-2F

CCTCCTGGGAGCTAAAGATA

248

HA – 1-2R

CGATCAGACCCTACAGGA

 

HA – 4-1F

CTTTGAGTGTACAGTGGATATAGAA

200

HA – 4-1R

AAAGATATGAGTAGGTAAGGCACA

 

HA – 4-2F

TGATAAAGTCTTCCCTGGTG

209

HA – 4-2R

TGCTTATTTCATCTCAATCCT

 

HA – 7-1F

CCTAGCAAGTGTTTTCCATT

246

HA – 7-1R

AGGTCCATCAAGAGTGTTTG

 

HA – 7-2F

GCCACAGGAAATCAGTCTAT

236

HA – 7-2R

TTCATTTTAAAGATCCAAGA

 

HA – 8-1F

TGAGCCAATTCAATCTCTTT

237

HA – 8-1R

ATCATCAAACCTGACCACAT

 

HA – 8-2F

TAATGAAGAAGCGGAAGACT

245

HA – 8-2R

TTTTGAGTATGGGGAAGAGA

 

HA – 9F

CCCAACCTCTCATCTTTTT

250

HA – 9R

CCAGACTTTTTCTTCTTACCTG

 

HA – 11-1F

CAGATTTGTAGAACCCTTGC

216

HA – 11-1R

TAGAGTAATAGCGGGTCAGG

 

HA – 11-2F

CTGCCAGGAGAAATATTCAA

239

HA – 11-2R

AAGGGGACATACACTGAGAA

 

HA – 13-1F

CATGACAATCACAATCCAAA

244

HA – 13-1R

AGAATGGGAATAGGGTGAGT

 

HA – 13-2F

GAGGTGGCATACTGGTACAT

219

HA – 13-2R

ATACGAATGGCTAGTGAAGC

 

HA – 14-1F

CTGTGTTATGAGTAACCAGAGT

243

HA – 14-1R

CCTAGTGCTAGGGTGTCTTG

 

HA – 14-2F

AATGCCATTGAACCAAGA

238

HA – 14-2R

CATATTTGGCTTCTTGGAGA

 

HA – 14-3F

TCTTGCGACAGAGTCCTACT

242

HA – 14-3R

TTTCTTCAACTCTGTTGCTG

 

HA – 14-4F

TCAGGCCTCCAATTAAGAT

250

HA – 14-4R

GGTCCACCAGACTCAGTAAG

 

HA – 14-5F

AGA TAC CAC TCT ATT TGG CA

229

HA – 14-5R

AAC TTT GAA TAA GGC ATT A

 

HA – 14-6F

TGGTAGGTTATTTAAAGGGAAA

248

HA – 14-6R

GCATTCTGTCATGAATCAAA

 

HA – 14-7F

CCATCAGTCTGGCAAAATA

250

HA – 14-7R

ATCCACCTTGCTGATTCTG

 

HA – 14-8F

CACAAAATCCAGATATGTCGT

235

HA – 14-8R

CCATCTCTTTGAGTCCTACG

 

HA – 14-9F

AGGAAAGGGTGAATTTACAA

249

HA – 14-9R

TTTTGCCTAGTGCTCAGTAA

 

HA – 14-10F

TTGCCTCAGATACATACAGTG

250

HA – 14-10R

TGCATGCATATTTCTCTACAA

 

HA – 14-11F

AAAACTTGGAAGGCTTGG

250

HA – 14-11R

TTGTAGTCTATCTGTGTGAGG

 

HA – 14-12F

GGTCCAAAAACATGAAACAT

245

HA – 14-12R

ATAAGATGCTGCTGGAAGAT

 

HA – 14-13F

GGTCCTATTCCAAGACAACTC

250

HA – 14-13R

AGATGTTTTGGGCAAGTCT

 

HA – 14-14F

CACATACAAGAAAGTTGAGAACA

250

HA – 14-14R

GCTTTCTGTTGCTACTCTCAG

 

HA – 14-15F

TGGAATGAAGCAAACAGAC

250

HA – 14-15R

TTTGTCCCTCATTTATTGCT

 

HA – 14-16F

ATACCATTTTGTCCCTGAAC

249

HA – 14-16R

TGTCAAAATCTTCCTTCTTCA

 

HA – 14-17F

TTCAGTCAGATCAAGAGGAAA

246

HA – 14-17R

TCAAATGTCACAAGAGCAGA

 

HA – 15F

TATTGCTTTTCCTCTGCTTT

214

HA – 15R

TTTCTTGTAATTCCACTGTCC

 

HA – 16-1F

GGGATGTAAACCCTAAGGAC

228

HA – 16-1R

ATGATGTTGCACTTTCCAA

 

HA – 16-2F

CTCTCGTCCCTATTCCTTCT

232

HA – 16-2R

TAAACCAAAAAGTGGTCAGC

 

HA – 17-1F

ACTCATAGGATTGATGTCTTCC

238

HA – 17-1R

TAAAAGTGGGATCTTCCATC

 

HA – 17-2F

CTAACACACTGAACCCTGCT

241

HA – 17-2R

TCATTTGTCAAAGTGCAATC

 

HA – 18F

TTTTAACAGGCTTCTCTGTG

246

HA – 18R

AGGTAGAAGAAAGAGCACAAAC

 

HA – 19F

AAATAATTTCTGTTCCTGTTG

222

HA – 19R

ATTCCAGAAAGGAAGAAAGC

 

HA – 25-1F

GGT GAC CAA GAG GCT AC

170

HA – 25-1R

GAC TGC TGG AGA TGA GGA

 

HA – 25-2F

CAG GGA GTA AAA TCT CTG C

155

HA – 25-2R

TGG TAT TTT TTT TCT TTC TT

 

HA – 26-1F

AGAAGTGAGAAAAGCGTCTG

244

HA – 26-1R

ACCCTCAGTAGAGGTCCTG

 

HA – 26-2F

GAATTCACCCCCAGAGTT

234

HA – 26-2R

AGAAATGCAGGACTGATGAT

 
  1. Some of the primers used for HRM were redesigned by LightCycler Probe Design Software 2.0. The primers not listed were the same with table 1