|
Exon
|
Oligo (5' – 3')
|
product size
|
|---|
|
HA – 1-1F
|
CCACTGATAAAAAGGAAGCA
|
226
|
|
HA – 1-1R
|
GTGGAGAGCTCTATTTGCAT
| |
|
HA – 1-2F
|
CCTCCTGGGAGCTAAAGATA
|
248
|
|
HA – 1-2R
|
CGATCAGACCCTACAGGA
| |
|
HA – 4-1F
|
CTTTGAGTGTACAGTGGATATAGAA
|
200
|
|
HA – 4-1R
|
AAAGATATGAGTAGGTAAGGCACA
| |
|
HA – 4-2F
|
TGATAAAGTCTTCCCTGGTG
|
209
|
|
HA – 4-2R
|
TGCTTATTTCATCTCAATCCT
| |
|
HA – 7-1F
|
CCTAGCAAGTGTTTTCCATT
|
246
|
|
HA – 7-1R
|
AGGTCCATCAAGAGTGTTTG
| |
|
HA – 7-2F
|
GCCACAGGAAATCAGTCTAT
|
236
|
|
HA – 7-2R
|
TTCATTTTAAAGATCCAAGA
| |
|
HA – 8-1F
|
TGAGCCAATTCAATCTCTTT
|
237
|
|
HA – 8-1R
|
ATCATCAAACCTGACCACAT
| |
|
HA – 8-2F
|
TAATGAAGAAGCGGAAGACT
|
245
|
|
HA – 8-2R
|
TTTTGAGTATGGGGAAGAGA
| |
|
HA – 9F
|
CCCAACCTCTCATCTTTTT
|
250
|
|
HA – 9R
|
CCAGACTTTTTCTTCTTACCTG
| |
|
HA – 11-1F
|
CAGATTTGTAGAACCCTTGC
|
216
|
|
HA – 11-1R
|
TAGAGTAATAGCGGGTCAGG
| |
|
HA – 11-2F
|
CTGCCAGGAGAAATATTCAA
|
239
|
|
HA – 11-2R
|
AAGGGGACATACACTGAGAA
| |
|
HA – 13-1F
|
CATGACAATCACAATCCAAA
|
244
|
|
HA – 13-1R
|
AGAATGGGAATAGGGTGAGT
| |
|
HA – 13-2F
|
GAGGTGGCATACTGGTACAT
|
219
|
|
HA – 13-2R
|
ATACGAATGGCTAGTGAAGC
| |
|
HA – 14-1F
|
CTGTGTTATGAGTAACCAGAGT
|
243
|
|
HA – 14-1R
|
CCTAGTGCTAGGGTGTCTTG
| |
|
HA – 14-2F
|
AATGCCATTGAACCAAGA
|
238
|
|
HA – 14-2R
|
CATATTTGGCTTCTTGGAGA
| |
|
HA – 14-3F
|
TCTTGCGACAGAGTCCTACT
|
242
|
|
HA – 14-3R
|
TTTCTTCAACTCTGTTGCTG
| |
|
HA – 14-4F
|
TCAGGCCTCCAATTAAGAT
|
250
|
|
HA – 14-4R
|
GGTCCACCAGACTCAGTAAG
| |
|
HA – 14-5F
|
AGA TAC CAC TCT ATT TGG CA
|
229
|
|
HA – 14-5R
|
AAC TTT GAA TAA GGC ATT A
| |
|
HA – 14-6F
|
TGGTAGGTTATTTAAAGGGAAA
|
248
|
|
HA – 14-6R
|
GCATTCTGTCATGAATCAAA
| |
|
HA – 14-7F
|
CCATCAGTCTGGCAAAATA
|
250
|
|
HA – 14-7R
|
ATCCACCTTGCTGATTCTG
| |
|
HA – 14-8F
|
CACAAAATCCAGATATGTCGT
|
235
|
|
HA – 14-8R
|
CCATCTCTTTGAGTCCTACG
| |
|
HA – 14-9F
|
AGGAAAGGGTGAATTTACAA
|
249
|
|
HA – 14-9R
|
TTTTGCCTAGTGCTCAGTAA
| |
|
HA – 14-10F
|
TTGCCTCAGATACATACAGTG
|
250
|
|
HA – 14-10R
|
TGCATGCATATTTCTCTACAA
| |
|
HA – 14-11F
|
AAAACTTGGAAGGCTTGG
|
250
|
|
HA – 14-11R
|
TTGTAGTCTATCTGTGTGAGG
| |
|
HA – 14-12F
|
GGTCCAAAAACATGAAACAT
|
245
|
|
HA – 14-12R
|
ATAAGATGCTGCTGGAAGAT
| |
|
HA – 14-13F
|
GGTCCTATTCCAAGACAACTC
|
250
|
|
HA – 14-13R
|
AGATGTTTTGGGCAAGTCT
| |
|
HA – 14-14F
|
CACATACAAGAAAGTTGAGAACA
|
250
|
|
HA – 14-14R
|
GCTTTCTGTTGCTACTCTCAG
| |
|
HA – 14-15F
|
TGGAATGAAGCAAACAGAC
|
250
|
|
HA – 14-15R
|
TTTGTCCCTCATTTATTGCT
| |
|
HA – 14-16F
|
ATACCATTTTGTCCCTGAAC
|
249
|
|
HA – 14-16R
|
TGTCAAAATCTTCCTTCTTCA
| |
|
HA – 14-17F
|
TTCAGTCAGATCAAGAGGAAA
|
246
|
|
HA – 14-17R
|
TCAAATGTCACAAGAGCAGA
| |
|
HA – 15F
|
TATTGCTTTTCCTCTGCTTT
|
214
|
|
HA – 15R
|
TTTCTTGTAATTCCACTGTCC
| |
|
HA – 16-1F
|
GGGATGTAAACCCTAAGGAC
|
228
|
|
HA – 16-1R
|
ATGATGTTGCACTTTCCAA
| |
|
HA – 16-2F
|
CTCTCGTCCCTATTCCTTCT
|
232
|
|
HA – 16-2R
|
TAAACCAAAAAGTGGTCAGC
| |
|
HA – 17-1F
|
ACTCATAGGATTGATGTCTTCC
|
238
|
|
HA – 17-1R
|
TAAAAGTGGGATCTTCCATC
| |
|
HA – 17-2F
|
CTAACACACTGAACCCTGCT
|
241
|
|
HA – 17-2R
|
TCATTTGTCAAAGTGCAATC
| |
|
HA – 18F
|
TTTTAACAGGCTTCTCTGTG
|
246
|
|
HA – 18R
|
AGGTAGAAGAAAGAGCACAAAC
| |
|
HA – 19F
|
AAATAATTTCTGTTCCTGTTG
|
222
|
|
HA – 19R
|
ATTCCAGAAAGGAAGAAAGC
| |
|
HA – 25-1F
|
GGT GAC CAA GAG GCT AC
|
170
|
|
HA – 25-1R
|
GAC TGC TGG AGA TGA GGA
| |
|
HA – 25-2F
|
CAG GGA GTA AAA TCT CTG C
|
155
|
|
HA – 25-2R
|
TGG TAT TTT TTT TCT TTC TT
| |
|
HA – 26-1F
|
AGAAGTGAGAAAAGCGTCTG
|
244
|
|
HA – 26-1R
|
ACCCTCAGTAGAGGTCCTG
| |
|
HA – 26-2F
|
GAATTCACCCCCAGAGTT
|
234
|
|
HA – 26-2R
|
AGAAATGCAGGACTGATGAT
| |
- Some of the primers used for HRM were redesigned by LightCycler Probe Design Software 2.0. The primers not listed were the same with table 1
