Table 1 Primer sequences and PCR conditions for LOXL1 sequencing
Exon | Primer Sequence | MgCl2 (mM) | Annealing Temperature (°C) | Amplicon (bp) |
|---|---|---|---|---|
1A | F: TCCCAGCCTGTTGCTTATTC | 1.5 | 55 | 326 |
| Â | R: AGGCCTGGTGGACAGAGAG | Â | Â | Â |
1B | F: AAGCAAGGAGCCTTCCTGTC | 1.5 | 55 | 260 |
| Â | R: GTACACCTGCCCGTTGTTCT | Â | Â | Â |
1C* | F: GCAGGTGTACAGCTTGCTCA | 1.5 | 55 | 464 |
| Â | R: ACACGAAACCCTGGTCGTAG | Â | Â | Â |
1D | F: CTCCTACCCGCAGCAGTTC | 1.5 | 55 | 227 |
| Â | R: GGTACTCGGGCAGCTCTTC | Â | Â | Â |
1E | F: CGACCAGGGTTTCGTGTACT | 1.5 | 55 | 280 |
| Â | R: AGGGTAGGCCTGCTCGAAG | Â | Â | Â |
1F | F: AGCAGGCCTACCCTGACC | 1.5 | 55 | 340 |
| Â | R: GCCTCCAGGAAGTTCTAAGGA | Â | Â | Â |
2 | F: GGAGGTCTCTGGGCATTAGC | 1.5 | 58 | 354 |
| Â | R: TCACTGATGAAACGGTCAGG | Â | Â | Â |
3 | F: TCTTCAGGGACAAGGAGTGG | 1.5 | 58 | 357 |
| Â | R: AGTGCTCATGGTGGGAGG | Â | Â | Â |
4 | F: CAGGGAAGACTAGGCCCTCT | 1.5 | 58 | 324 |
| Â | R: CTGTGAGCAGAGCTGAGTGG | Â | Â | Â |
5 | F: GAAACAAGCAGCATCACAGC | 1.5 | 58 | 220 |
| Â | R: GGCTGAAGCTTCTTTCAGGA | Â | Â | Â |
6 | F: TCTGGTGAGCAGTTGAGGTG | 1.5 | 58 | 202 |
| Â | R: GGTGGGCAAACTCTTACTGG | Â | Â | Â |
7 | F: CCCTCATTGACCCACTGTCT | 1.5 | 58 | 356 |
| Â | R: GCATGCAGAGCCACAGAGTA | Â | Â | Â |