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Table 1 Sequences of primers and PCR products length

From: Distribution of the FYB ES and RHCE*ce(733C>G) alleles in an Argentinean population: Implications for transfusion medicine

Analysis of

Primers

5'-3' nucleotide sequencea

Product length

FY alleles

Fy normal 2

ccctcattagtccttggctcttct

713 bp

 

Fy null

ccctcattagtccttggctctttc

 
 

FyA4

CAGCTGCTTCCAGGTTGGCTC

 
 

FyB4

CAGCTGCTTCCAGGTTGGCTT

 

Hybrid Rhesus box

5' IR

tcctgcagcaaacttctga

1980 bp

 

3' IR

tctcttttctggccttaacatc

 

RHCE*ce(733C>G) allele

RHCEint4F1

agactgctgggagaggctaat

807 bp

 

733G rev

CACCACGCTGACTGCTAC

 
 

733C rev

CACCACACTGACTGCTAG

 

HLA Class II DRB1 exon 2

2DRBAmpA

ccgaccacgacgtttcttg

274 bp

 

2DRBAmpB

ccgctgcactgtgaagctctc

 
  1. a. Capital letters denote nucleotides in exons while small letters denote nucleotides in non-coding sequences. Intentionally mismatched bases are underlined.