Figure 5

FAM5C is expressed in aortic endothelial and smooth muscle cells. (A) RT-PCR was performed on RNA derived from the cerebellum (control) and proliferating aortic endothelial (HAEC, passage 6) and smooth muscle cells (AoSMC, passage 5 and 8). (B) Real time RT-PCR was performed on RNA isolated from serially passed AoSMC. Aliquots were taken at every passage and experiments were performed in triplicate. FAM5C and GAPDH expression were assayed at the same time and GAPDH was used to normalize all samples. The expression level was calculated relative to heart RNA (control). Standard deviation was calculated for each experiment and is indicated by grey bars.