Table 2 Sequences of used primers and used restrictases.
From: Gene polymorphisms of superoxide dismutases and catalase in diabetes mellitus
SNP | sequence of used primers | restriction endonuclease | restriction fragments |
|---|---|---|---|
SOD1 35 A/C | 5'CTATCCAGAAAACACGGTGGGCC 3' | HhaI | C allele 71 bp and 207 bp A allele 278 bp |
| Â | 5'TCTATATTCAATCAAATGCTACAAAAC3' | Â | Â |
SOD2 A16V (C/T) | 5'GCTGTGCTTTCTCGTCTTCAG 3' | BsaWI | C allele 267 bp T allele 183 bp and 84 bp |
| Â | 5'TGGTACTTCTCCTCGGTGACG3' | Â | Â |
CAT -21 A/T | 5'-AATCAGAAGGCAGTCCTCCC-3' | HinfI | A allele 203 bp and 47 bp T allele 250 bp |
| Â | 5'-TCGGGGAGCACAGAGTGTAC-3' | Â | Â |