Skip to main content

Table 3 Minisequencing primers

From: Minisequencing mitochondrial DNA pathogenic mutations

POSITIONS

Extension Primer1

Length

Base change

Strand

Final Concentration (μM)3

10158

acaactcaacggctacatagaaaaa

25

T>C

L

0.2

3946

Cgaactagtctcaggcttcaacatc

25

G>A

L

0.2

10663

GACTGcaatattgtgcctattgccatactag

31

T>C

L

0.2

3460

(GACT)2Ggctactacaacccttcgctgac

31

G>A

L

0.2

10191

(GACT)4agtgcggcttcgaccctata

36

T>C

L

0.3

14459

(GACT)2GACctcaggatactcctcaatagccatc

36

G>A

L

0.3

144842

(GACT)3atcgctgtagtatatccaaagacaacYa

40

T>C

L

0.5

3243

(GACT)4GAacagggtttgttaagatggcag

40

A>G

L

0.2

11777

(GACT)5Gcaaactacgaacgcactcacagt

44

C>A

L

0.2

7445

(GACT)5tcgaagaacccgtatacataaaatctag

48

A>G/C

L

0.2

12706

(GACT)9gcggtaactaagattagtatggtaattagga

52

T>C

H

0.2

39492

(GACT)11GAagtctcaggcttcaacatcRaa

52

T>C

L

0.2

144872

(GACT)7GctgtagtatatccaaagacaaccaSca

56

T>C

L

0.6

13513

(GACT)8ttcctcacaggtttctactccaaa

56

G>A

L

0.1

13514

(GACT)10Ttgcggtttcgatgatgtgg

60

A>G

H

0.2

3697

(GACT)9CTaaactcaaactacgccctgatc

60

G>A

L

0.2

11778

(GACT)9GAgaagtccttgagagaggattatgatg

64

G>A

H

0.3

11832

(GACT)8 GACtcaaactctactcccactaatagcttttt

64

G>A

L

0.1

9176

(GACT)11Gatccaagcctacgttttcacacttc

70

T>C/G

L

0.2

14482

(GACT)11gccatcgtcgctgtagtatatccaaagacaac

73

C>A/G

L

0.6

8993

(GACT)13Gcctactcattcaaccaatagccc

76

T>G/C

L

0.2

  1. 1 Capital letters indicate the segment of the primer belonging to the tail
  2. 2 These primers are 'degenerated' at the indicated position in the second column (see Material and Methods) using the IUB code