N-acetyltransferase 8, a positional candidate for blood pressure and renal regulation: resequencing, association and in silico study

Table 3 Diversity parameters for resequenced NAT8 gene and promoter regions of 29 genes associated with blood pressure determination

Region	SNPs	π	θ	D^T
A. NAT8 region
Gene (1569 bp)	9	0.00037	0.00102	-1.58
mRNA (953 bp)	6	0.00045	0.00127	-1.49
Promoter (600 bp)^a	6	0.00320	0.00221	1.05
B. Promoter regions (600 bp)^a of 29 blood pressure associated genes^b
AGTR1	1	0.00082	0.00043	1.47
CYP17A1	2	0.00058	0.00043	0.57
CLCNKA	2	0.00081	0.00086	-0.11
CYP11B2	3	0.00168	0.00085	2.02
NPR2	1	0.00042	0.00043	-0.018
BSND	2	0.00023	0.00043	-0.74
SAH	2	0.00071	0.00087	-0.38
SGK	1	0.00079	0.00043	1.36
SLC12A3	1	0.00075	0.00043	1.216
SCNN1A	3	0.00167	0.00128	0.721
IL1A	2	0.0017	0.00086	2.02
SLC14A2	4	0.00133	0.00085	1.17

Abbreviations: π, estimate of nucleotide diversity per site from average pairwise difference among individuals; θ, estimate of nucleotide diversity per site from number of segregating sites; D ^T, value of Tajima's D statistics expressing the difference between π and θ estimates
^aRegion between -500 bp and +100 bp relative to mRNA transcription start point
^bNo SNPs were detected in Estonian sample by resequencing the promoter regions for ACE, ADD1, ADD2, AGT, AGTR2, KCNJ1, NPR1, HSD11B1, CLCNKB, KLK1, NR3C2, CYP21A2, SLC22A2, REN, ATP1A1, SCNN1B, SLC8A1 genes

ISSN: 1471-2350