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Table 1 SOD2 primer sequences and PCR conditions used for this study

From: SOD2polymorphisms: unmasking the effect of polymorphism on splicing

SOD2 exon

Primers (Top-forward primer, Middle-reverse primer, Bottom-sequencing primer)

Size of amplicon

Tm (C°)

Mg2++ (mM)

1&2

A-GTAGCACCAGCACTAGCAGGA

713

55

2

 

B-AAGCGAGTTCTCCTCCCGGAGA

   
 

P-GTCCACTGTCGCCATTG (for exon 1)

   
 

Q-GTGGTACGCTGACTGAC (for exon 2)

   

3

K-CCAGGTGTCGCATTCTGATGTTG

362

55

2

 

D-CAGTAGAGCATCTCTCCCAAATG

   
 

C-GAAATCTGTTCATTTGTGGGTGG

   

4

E-GCTGGTCCCATTATCTAATAGC

277

55

2

 

F-CAATCGATTCCTACTGTGCAC

   
 

L-CAGTGGTTGAAAAAGTAGGAG

   

5

G-TTAGACTGAAACTGATGGTTGG

217

55

2

 

H-CATCTCAGCATAACGATCGTGG

   
 

M-GCAAGCCATGTATCTTTCAG

   
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BMC Medical Genetics

ISSN: 1471-2350

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