Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro

Table 1 PCR primers and conditions for DHPLC analysis of DSC2 gene

EXON	bp	T°C DHPLC	Forward 5' 3'	Reverse 5' 3'
1+5'UTR	708	Direct sequencing	TCAGACCTCGCTCTGTAATTGA	TATCCCCGTTCCCCTAGTTT
2	199	Direct sequencing	ACACATTAAAGTTTTCTTTTTAT	GGCGTATATGTACCACAGCA
3	400	54,8/55,4	CCCCACGTGCATACATTACT	TGGTTTTCATTCGTCTTTAAGC
4	269	55,6/58.5	CCCCTACCCAGCTAATCCTC	GGAAACTATAGACTCCCACAGCA
5	332	56,5/57,2	TGAAAGCTCTGCTGAAATAAAGA	GGAGTAGCCAGAGCATTGGT
6	266	54.7	GCCAAAATGAATTTGAAGCATAC	TTGAAACACAGTTAATTTGCCATA
7	384	55/58/59,5	CATAGAACATGTGAATGTTTTGGA	CAAAACCAGCATACTCCAAGG
8	252	54,3/55.6	GTTGGTGCTTTCCCCCAATA	AGGCCAGAGATGTGCATATTA
9	324	52.2/53.5/55.3	CATCGTGTTCAATTTTTGTGA	CCTTTCTTTCCATTAAATTCTAGC
10	374	56,5/57,9	ACTCGTTAGCATTGCCAAAT	TAACGTAACAAAATAAGCTA
11	375	51,7/56	CAAGAAGTAGCAGTGGCATAAGG	AACAGAGTGCATGTATCCAGCTT
12	345	57/58/59	GTGTTCAGTGCATACTTTTGTGG	GCAGACATCCTGATGTTGAAAA
13	356	57,2/58	TGTTCAGAAGAAATCAGTGACA	GTGTCTTGAAAGTTACTTTAAAGG
14	263	57.7	GATTTATGTGTGTATTAACCATTG	CGCATTATAAGCGAATTCATCC
15	a 194	56.2/60.6/61.8	CATAATTTTGTGTTCCTCTCTGT	AGGATTCCGAGGTCTGGTGT
	b 332	61/61,4/62,2	GGCTTCACAACCCAAACTGT	TGAAAATTATAGTCAGAATCCAGT
15'	226	53,7/55,2	GCCACATGCGTGACTTTTAG	ACTTTCTGCCAAGGGGAAAA
16	397	56,3/56,8	CAATGAAAGGTAAATCAAAGCAA	AAAAACCCCCACAAATAGCA

ISSN: 1471-2350