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Table 1 Primers and probe sequences and annealing temperatures used.

From: Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1–12 dosage

Gene/exon

Sequences

Tann

β-globin

Forward primer: 5'-GTGCACCTGACTCCTGAGGAGA-3'

Reverse primer: 5'-CCTTGATACCAACCTGCCCAG-3'

Probe: 5'-FAM-AAGGTGAACGTGGATGAAGTTGGTGG-RTQ1-3'

60°C

PARK2\ex1

Forward primer: 5'-CAGCGGCTCTCCTGGGTTAAA-3'

Reverse primer: 5'-TACGACTCCCAGCAGGCCCT-3'

Probe: 5'-ROX-CCTAGGAATGCGCACGCGCGGAG-RTQ1-3'

60°C

PARK2\ex2

Forward primer: 5'-ATCTCAGGCATGAATGTCAGATT-3'

Reverse primer: 5'-TGACCAGTTGCGTGTGATTT-3'

Probe: 5'-ROX-ATGAGCAATGGAGCTGGCGGCATCC-RTQ1-3'

60°C

PARK2\ex3

Forward primer: 5'-CATTGTGCAGAGACCGTGGAGAA-3'

Reverse primer: 5'-GCTGAGGTCCACCCGAGTCAA-3'

Probe: 5'-ROX-AAATGAATGCAACTGGAGGCGACGACCC-RTQ1-3'

60°C

PARK2\ex4

Forward primer: 5'-TAGCCACTTCTTCTGCTTTT-3'

Reverse primer: 5'-TGCACTGTACCCTGAGTTTT-3'

Probe: 5'-ROX-ATTGCAAAGGCCCCTGTCAAAGAGTGCA-RTQ1-3'

60°C

PARK2\ex5

Forward primer: 5'-TCTTGCTGGGATGATGTTTTAATTCCAAA-3'

Reverse primer: 5'-TTGCAATAAGAGGAATGAAT-3'

Probe: 5'-ROX-AATGCCAATCCCCACACTGCCCTGG-RTQ1-3'

60°C

PARK2\ex6

Forward primer: 5'-CCGTGGAGGGAAGTGACACTAT-3'

Reverse primer: 5'-TGCACCTGATCGCAACAAAT-3'

Probe: 5'-ROX-TGTGCGCGTAATGCAAGTGATGTTCCG-RTQ1-3'

60°C

PARK2\ex7

Forward primer: 5'-GGGTCGTGAACAAACTGCCGATCATT-3'

Reverse primer: 5'-AGGAGCCCCGTCCTGGTTTT-3'

Probe: 5'-ROX-AAGCAAATCACGTGGCGGGAGTTGCAC-RTQ1-3'

60°C

PARK2\ex8

Forward primer: 5'-TCACTCACCTGCTCTTCTCCCAGAAT-3'

Reverse primer: 5'-CAGAGTGAAAGTGACGTTTT-3'

Probe: 5'-ROX-TCAAGGAGTTGGGACAGCCAGCTGTTGG-RTQ1-3'

60°C

PARK2\ex9

Forward primer: 5'-ACCCCACAGCCCAGGCCATT-3'

Reverse primer: 5'-TGCAGATGGGGGGCGTGTTA-3'

Probe: 5'-ROX-TTCGCAGGTGACTTTCCTCTGGTCAGGC-RTQ1-3'

60°C

PARK2\ex10

Forward primer: 5'-GCTTGGAGGAATGAGTAGGGCATT-3'

Reverse primer: 5'-GTTTGCCTTCTGCCGGGAAT-3'

Probe: 5'-ROX-AGGCTTCAAATACGGCACTGCACTCCCC-RTQ1-3'

60°C

PARK2\ex11

Forward primer: 5'-TGGTGGTTTTCTTGATGGTTT-3'

Reverse primer: 5'-AACGCCTTTCCTCTTTGTTT-3'

Probe: 5'-ROX-TCGGCGGCTCTTTCATCGACTCTGTAGG-RTQ1-3'

60°C

PARK2\ex12

Forward primer: 5'-CACGATCTTCCTGAGAAGTCAGACAAT-3'

Reverse primer: 5'-ACAACACTGCATGCGGCAAA-3'

Probe: 5'-ROX-TCTGCGTAGTGTGGGTAAGAGCATGCGG-RTQ1-3'

60°C

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BMC Medical Genetics

ISSN: 1471-2350

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