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Table 5 Pyrosequencing primers, dispensation order and sequence to analyse for relevant assays

From: Resequencing of genes for transforming growth factor β1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy

Primer Set

Forward Primer

Reverse Primer

Sequence Primer

Dispensation order

Sequence to Analyse

TGFBR2 c.*747C>G

tcctgtgtgcccttatttctc

tgaaggtaaaaagtggggttc

agtttctaaactaggttgag

tcgagagtctac

c/ggagagtttctaaac

TGFBR2 c.1149G>A

gatcacactccatgtggg

ccagacgcagggaaagc

agagctccaatatcctc

tgatgagacgac tac

g/atgaagaacgacctaacc

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BMC Medical Genetics

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