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Table 4 TaqMan primers, probes, quencher and annealing temperature for relevant assays.

From: Resequencing of genes for transforming growth factor β1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy

Primer set

Primer 1

Primer 2

FAM™ Labelled Probe

Vic™ Labelled Probe

Fluorescent Quencher

Anneal (1 minute)

TGFB1 -800G>A

gctatcgcctgcacacagc

aggacagaagcggtcccat

tgcctccaacgtcaccaccatc

tctgcctccaacatcaccaccatc

TAMRA

62°C

TGFB1 -509C>T

ttagccacatgggaggtgct

ccaggcggagaaggcttaa

acccttccatccctcaggtgtcct

ccctcccatccttcaggtgtcctg

TAMRA

62°C

TGFB1 +869T>C

caccacaccagccctgttc

ccaggcgtcagcaccagta

agcagcggcagca

cagcagcagcagc

None

60°C

TGFB1 +915G>C

Developed by Applied Biosystems (USA) as a Research and Development Kit

None

56°C

  1. TGFBR1+72InsC was genotyped by a commercial Invader kit designed and manufactured by Third WAVE technologies (Madison, MI, USA)