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Table 3 WAVE conditions used for screening TGFBR1 and TGFBR2 genes.

From: Resequencing of genes for transforming growth factor β1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy

Primer Set Tm°C Melt Range°C Run Temperature°C WAVE Buffer B (%)
TGFBR1 i 59.4 54 – 72 57 69–79
    59 67–77
    64 62–64
TGFBR1 ii 63.3 60 – 72 63 68–78
    69 62–72
TGFBR1 iii 69.2 58 – 75 69 68–78
    72 65–75
TGFBR1 iv 55.9 54 – 65 55 68–78
    59 64–74
TGFBR1 v 57.2 54 – 65 55 62–72
    59 58–68
    61 56–58
TGFBR1 vi 56.1 54 – 65 56 68–78
    59 65–75
TGFBR1 vii 56.5 54 – 68 55 69–79
    58 66–76
TGFBR1 viii 57.0 54 – 65 57 67–77
TGFBR1 ix 57.4 55 – 67 56 67–77
    58 64–75
    62 61–71
TGFBR1 x 54.8 51 – 66 54 65–75
    57 62–72
TGFBR1 xi 54.6 54 – 67 54 65–75
    58 60–70
TGFBR1 xii 56.9 55 – 65 56 64–77
    58 62–72
TGFBR1 xiii 55.8 52 – 62 56 67–77
TGFBR1 xiv 55.2 54 – 63 55 67–77
    59 63–73
TGFBR2 i 66.1 59–74 60 63–73
    65 58–68
    67 56–66
    70 53–63
TGFBR2 ii 66.3 61 – 72 64 58–68
    66 56–66
    69 52–62
TGFBR2 iii 58.5 57 – 61 59 61–71
    61 59–69
TGFBR2 iv 59.8 55 – 65 56 67–77
    61 60–70
    63 58–68
TGFBR2 v 60.5 57–64 62 58–68
TGFBR2 vi 63.5 62–65 64 55–65
TGFBR2 vii 59.7 53–71 59 57–67
    61 52–62
TGFBR2 viii 59.7 53 – 71 59 66–76
    61 64–74
TGFBR2 ix 61.0 60–61 61 57–67
TGFBR2 x 57.0 51–66 52 63–73
    58 57–67
    62 53–63
    64 50–60
TGFBR2 xi 54.4 53 – 66 54 67–77
    56 65–75
    60 61–71
TGFBR2 xii 57.0 56–60 59 63–73