Figure 2From: Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene Analysis of SNP BC+1 within intron BC of ADAM33. (A) Schematic showing the location of SNP BC+1 within intron BC and the protective (Pr) and at-risk (Ar) alleles A and G respectively. (B) Schematic defining the constructs generated to analyze the function of the two alleles A and G. The PCR generated fragments were cloned into the multiple cloning site at the NheI (N) and BglII (B) restriction sites of the pSEAP2 Basic vector.Back to article page