Table 2 Extension primer sequences
Mutation | Extension Primer | Size bp | Change |
|---|---|---|---|
BRCA1 c.187-188delAG | F gactcattaatgctatgcagaaaatcttag | 30 | A/T |
BRCA1 c.5236 G > C | F gacttgaacggacactgaaatattttctag | 30 | G/C |
BRCA1 c.330 A > G | F gactgatgtcctttatgtaagaatgatataaccaaa | 36 | A/G |
BRCA2 c.5374-5377delTATG | F gactgactgactaatactgcagattatgtaggaaattatttg | 42 | T/A |
BRCA1 c.589-590delCT | F gactgactgactgactgactgactaaaccagtctcagtgtccaactct | 48 | C/A |
BRCA2 c.3036-3039delACAA | F gacgactgactgactgactgactgactggttttatatggagacacaggtgataa | 54 | A/G |
BRCA1 c.5242C > A | R acgactgactgactgactgactgactgactgactgactgctaactacccattttcctccc | 60 | T/G |
BRCA2 c.9254-9258del5 | F gacgactgactgactgactgactgactgactgttaacagaaggaaagagatacagaattt | 60 | A/C |
BRCA2 c.6857-6858delAA | F gacgactgactgactgactgactgactgactgactgactctgatgttcctgtgaaaacaaatatag | 66 | A/G |
BRCA2 c.9538-9539delAA | F gagactgactgactgactgactgactgactgactgactgactgactacgaatgttacaatttactggcaata | 72 | A/G |