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Table 1 Sequence primers used for in vitro mutagenesis.

From: Functional analysis of splicing mutations in exon 7 of NF1gene

Mutation

Mutagenic primer plus

Mutagenic primer minus

R304X

5'-AAGTTATTTCTGGACAGTCTATGAAAAGCTCTTGCTGGCCATGG-3'

5'-CCATGGCCAGCAAGAGCTTTTCATAGACTGTCCAGAAATAACTT-3'

Q315Q

5'-GGCCATGGAGGAAGTAGGCAACTGACAGAAAGTGCTGCAATTG-3'

5'-CAATTGCAGCACTTTCTGTCAGTTGCCTACTTCCTCCATGGCC-3'

L316M

5'-GGCCATGGAGGAAGTAGGCAGATGACAGAAAGTGCTGCAATTG-3'

5'-CAATTGCAGCACTTTCTGTCATCTGCCTACTTCCTCCATGGCC-3'

Q315Q+L316M

5'-GGCCATGGAGGAAGTAGGCAAATGACAGAAAGTGCTGCAATTG-3'

5'-CAATTGCAGCACTTTCTGTCATTTGCCTACTTCCTCCATGGCC-3'

N335N

5'-GTAAAGCAAGTACTTACATCAACTGGGAAGATAACTCTGTC-3'

5'-GACAGAGTTATCTTCCCAGTTGATGTAAGTACTTGCTTTAC-3'