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Table 1 Sequence primers used for in vitro mutagenesis.

From: Functional analysis of splicing mutations in exon 7 of NF1gene

Mutation Mutagenic primer plus Mutagenic primer minus
R304X 5'-AAGTTATTTCTGGACAGTCTATGAAAAGCTCTTGCTGGCCATGG-3' 5'-CCATGGCCAGCAAGAGCTTTTCATAGACTGTCCAGAAATAACTT-3'
Q315Q 5'-GGCCATGGAGGAAGTAGGCAACTGACAGAAAGTGCTGCAATTG-3' 5'-CAATTGCAGCACTTTCTGTCAGTTGCCTACTTCCTCCATGGCC-3'
L316M 5'-GGCCATGGAGGAAGTAGGCAGATGACAGAAAGTGCTGCAATTG-3' 5'-CAATTGCAGCACTTTCTGTCATCTGCCTACTTCCTCCATGGCC-3'
Q315Q+L316M 5'-GGCCATGGAGGAAGTAGGCAAATGACAGAAAGTGCTGCAATTG-3' 5'-CAATTGCAGCACTTTCTGTCATTTGCCTACTTCCTCCATGGCC-3'
N335N 5'-GTAAAGCAAGTACTTACATCAACTGGGAAGATAACTCTGTC-3' 5'-GACAGAGTTATCTTCCCAGTTGATGTAAGTACTTGCTTTAC-3'