Figure 2
From: Functional analysis of splicing mutations in exon 7 of NF1gene
Representation of the pSPL3-NF1-7 minigene construct. Genomic sequence from exon 7 of the NF1 gene along with 258 bp of 5' and 248 bp of 3' intronic flanking sequence was ligated into pSPL3 plasmid. Shaded sequences describe the nucleotide changes introduced independently by in vitro mutagenesis to obtain the mutated constructs.