BMC Medical Genetics

Table 1 Primers used in PCR/sequencing reaction of WFS1 coding region*

From: A novel mutation in the WFS1gene identified in a Taiwanese family with low-frequency hearing impairment

Primer sequence
Fragment	Forward primer (5-3)	Reverse primer (5-3)
Exon 2^†	AAGCGGTGCTGGCCCATG	CCGTTCCCACCCAGCTATC
Exon 3^†	TACTCCTGGCCTGGATTTGA	CATGGGCACCCTACCAACA
Exon 4^†	AGTGGCCGGAGGCTCAGT	CCAACAGCATCACCAGCGT
Exon 5^†	AGTCAGATGTCCATGCATCC	CTCTACAGGAAGGTTCTGGT
Exon 6^†	GAGCACGCTACGTGGTGCT	GGAGGCACGGGTGAGATAG
Exon 7^†	CCTGAACCCACTCAGCTC	CCAGCGGCACGGCTGTAA
Exon 8-1^§	TTCCCACGTACCATCTTTCC	CACATCCAGGTTGGGCTC
Exon 8-2^§	AGAACTTCCGCACCCTCAC	TCAGGTAGGGCCAATTCAAG
Exon 8-3^§	CTATCGCTGCTGCCCTCC	GGGCAAAGAGGAAGAGGAAG
Exon 8-4^§	GTGAGCTCTCCGTGGTCATC	CCCTCTGAGCGGTACACATAG
Exon 8-5^§	ATCCTGGTGTGGCTCACG	GTAGAGGCAGCGCATCCAG
Exon 8-6^§	GCGTGACTGACATCGACAAC	GCTGAACTCGATGAGGCTG
Exon 8-7^§	CAGCAGCGAGTTCAAGAGC	CCTCATGGCAACATGCAC

*WFS1 sequence based on the GenBank entry (accession number: AC004599).
^†Primers were designed and synthesized herein (Methods).
^§Primers were provided by Dr. Yann-Jinn Lee.

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ISSN: 1471-2350

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