Figure 4
Expression and functional studies of methylmalonyl-CoA mutase in primary mut ohuman hepatocytes. A. Western analysis of protein lysates from primary human hepatocytes probed with methylmalonyl-CoA mutase antibody (78 kDa, labeled mutase) and cross-reactive beta-actin loading control (47 kDa, labeled actin). Lane 1: human control whole liver extract (20 micrograms) Lane 2: mut owhole liver extract (20 micrograms); Lane 3: human primary mut oprimary human hepatocytes (10 micrograms); Lane 4: adenoviral corrected mut oprimary human hepatocytes (10 micrograms). B. Human hepatocytes (wild type, mut oand mut ocorrected with adenovirus) were assayed for [1-14C]-propionate incorporation over 18 hours after viral incubation for 48 hrs. Activity was normalized to total protein content of the extracts. The samples were analyzed in triplicate. The bars around the average represent +/- one standard deviation.