Polymorphism | Primer sequence | Product size (bp) | Annealing temp./restriction enzyme/allele sizes |
---|---|---|---|
TGFβ1 G>A (-800) Promoter | F: 5' GGCAGTTGGCGAGAACAGT-3' R: 5' ACCCAGAACGGAAGGAGAGT3' | 600 | 57°C/Tai I/ G = 199, 401 A = 600 |
TGFβ1 C>T (-509) Promoter | F: 5'-GGCAGTTGGCGAGAACAGT-3' R: 5'-ACCCAGAACGGAAGGAGAGT-3' | 600 | 57°C/Eco 8I1/ C = 489,111 T = 600 |
TGFβ1 Arg25Pro Exon 1 | F: 5'TTC CCT CGA GGC CCT CCT A3' R: 5' CAC AGC AGC GGT AGC AGC TG3' | 294 | 61°C/Sac II/ G = 202,67,25 C = 269,25 |
TGFβ1 Tyr81His Exon 2 | F: 5' CCAGATCCTGTCCAAGCTG3' R: 5' TGGGTTTCCACCATTAGCAC3' | 198 | 57°C/Rsa I/ T = 89,109 C = 198 |
TGFβ1 Thr263Ile Exon 5 | F: 5' CACCAAAGCAGGGTTCACTA3' R: 5' ATCCAGGCTACAAGGCTCA3' | 238 | 58°C/Fok I/ C = 238 T = 84, 154 |
TNFα G>A -308 Promoter | F:5'AGGCAATAGGTTTTGAGGGcCAT3' R:5'GGGACACACAAGCATCAAGGATAC3' | 146 | 37°C/Nco I/ G = 146 A = 122, 24 |
CCR2 Val64Ile Exon 1 | F: 5'TTG TGG GCA ACA TGA TGG3' R: 5'GCA TTC CCA AAG ACC CAC TC3' | 163 | 57°C/BsaBI/ T = 163 C = 145,18 |
CCR5 G>A (59029) Promoter | F: 5' CAG TCA ACC TGG GCA AAG CC3' R: 5' AGC TTT GGT CCT GAG AGT CC3' | 453 | 57°C/Bst 1286I/ G = 408, 45 A = 453 |
CCR5 Δ32 Promoter | F:5' GAAGTTCCTCATTACACCTGCAGCTCTC3' R:5' CTTCTTCTCATTTCGACACCGAAGCAG AG3' | 174/142 | 55°C/ 1 = 174 2 = 142 |