Skip to main content

Table 1 SNPs in TGFβ1, TNFα, CCR2 and CCR5 genes, their location, primer sequences, PCR conditions and restriction enzyme with product sizes.

From: Association of TGFβ1, TNFα, CCR2 and CCR5 gene polymorphisms in type-2 diabetes and renal insufficiency among Asian Indians

Polymorphism

Primer sequence

Product size (bp)

Annealing temp./restriction enzyme/allele sizes

TGFβ1

G>A (-800)

Promoter

F: 5' GGCAGTTGGCGAGAACAGT-3'

R: 5' ACCCAGAACGGAAGGAGAGT3'

600

57°C/Tai I/

G = 199, 401

A = 600

TGFβ1

C>T (-509)

Promoter

F: 5'-GGCAGTTGGCGAGAACAGT-3'

R: 5'-ACCCAGAACGGAAGGAGAGT-3'

600

57°C/Eco 8I1/

C = 489,111

T = 600

TGFβ1

Arg25Pro

Exon 1

F: 5'TTC CCT CGA GGC CCT CCT A3'

R: 5' CAC AGC AGC GGT AGC AGC TG3'

294

61°C/Sac II/

G = 202,67,25

C = 269,25

TGFβ1

Tyr81His

Exon 2

F: 5' CCAGATCCTGTCCAAGCTG3'

R: 5' TGGGTTTCCACCATTAGCAC3'

198

57°C/Rsa I/

T = 89,109

C = 198

TGFβ1

Thr263Ile

Exon 5

F: 5' CACCAAAGCAGGGTTCACTA3'

R: 5' ATCCAGGCTACAAGGCTCA3'

238

58°C/Fok I/

C = 238

T = 84, 154

TNFα

G>A -308

Promoter

F:5'AGGCAATAGGTTTTGAGGGcCAT3'

R:5'GGGACACACAAGCATCAAGGATAC3'

146

37°C/Nco I/

G = 146

A = 122, 24

CCR2

Val64Ile

Exon 1

F: 5'TTG TGG GCA ACA TGA TGG3'

R: 5'GCA TTC CCA AAG ACC CAC TC3'

163

57°C/BsaBI/

T = 163

C = 145,18

CCR5

G>A (59029)

Promoter

F: 5' CAG TCA ACC TGG GCA AAG CC3'

R: 5' AGC TTT GGT CCT GAG AGT CC3'

453

57°C/Bst 1286I/

G = 408, 45

A = 453

CCR5

Δ32

Promoter

F:5' GAAGTTCCTCATTACACCTGCAGCTCTC3'

R:5' CTTCTTCTCATTTCGACACCGAAGCAG AG3'

174/142

55°C/

1 = 174

2 = 142