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Figure 1 | BMC Medical Genetics

Figure 1

From: The GCC repeat length in the 5'UTR of MRP1 gene is polymorphic: a functional characterization of its relevance for cystic fibrosis

Figure 1

Comparison of the transcriptional activity of MRP1 alleles containing 7 or 14 GCC triplets. 16HBE14o-AS3 cells were transfected with reporter vectors including the MRP1 5'UTR region with 7 [MRP1(GCC)7-luc] or 14 triplets [(GCC)14-luc] driving the luciferase gene. 16HBE14o-AS3 cells transfected with MRP1(GCC)7-luc and not treated with AZM were considered as calibrator. Percentages of luciferase activity normalized with beta galactosidase are shown. Specificity of the signal was assessed by determining the luciferase activity in insert-less (pGL3) transfected cells, which was almost undetectable. Experiments were performed in triplicate (n = 3, p > 0.05 for all conditions, paired T-Test; SEMs are indicated by bars).

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BMC Medical Genetics

ISSN: 1471-2350

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