Figure 2

Quantitative RT-PCR (qPCR) analysis of Spock1 expression. Quantitative RT-PCR analysis of the expression of (A.) human SPOCK1 in the clonal lymphocyte RNAs (Mt Ly, Wt Ly) used for the microarray experiments and of mouse Spock1 in differentiating wild-type (Wt EB) and Mecp2 ^R308/Ymutant (Mt EB) male mouse embryoid bodies; (B.) cerebral cortex (Wt Cx, Mt Cx), cerebellum (Wt Cb, Mt Cb) and olfactory bulb (Wt OB, Mt OB) of adult (5 week-old) wild-type and Mecp2 ^R308/Ymutant male mice; and (C.) E16.5 embryo head (Wt head, Mt head) and body (Wt body, Mt body) (4 independent samples were tested for each tissue and genotype). The Y-axis shows the average fold change in expression +/- standard deviation) of mutant compared to wild-type, which is set as one-fold baseline, except for the Ly samples, where the one-fold baseline was set in the mutant for figure clarity (light bars = wild-type; dark bars = mutant), p-values are given under each respective graph.