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Table 1 Primers and DGGE screening conditions

From: Three novel and the common Arg677Ter RP1 protein truncating mutations causing autosomal dominant retinitis pigmentosa in a Spanish population

Primers* cDNA Sequence Fragment size (bp) Gradient %** Running T (°C)
Forward 5'-GTAATAACTCTGGAACTGACAA-3' 1998–2308 350 40–70 50
Reverse 5'-GC-GGACTATCTGAACTTTGCACTA-3'     
Forward 5'-CAGGTATCAAGATGGACAGC-3' 2197–2461 304 40–70 50
Reverse 5'-GC-TGAACCTTGGAATTTTGAGTAG-3'     
  1. * (GC) = 5'-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCG-3' ** 100% denaturant = 7M urea and 40% (v/v) formamide in TAE buffer