Table 1 PCR and sequencing primers for the OAZ3 gene. An "F" in the name designates a forward primer, an "R" designates a reverse primer. The position given for each primer is that of the first nucleotide, in relation to the first nucleotide of the ATG start codon.
Region | Â | Â | Â | Â |
|---|---|---|---|---|
Primer positions | Â | Â | Â | Â |
PCR size | Name | PCR Primers 5'-3' | Name, Position | Sequencing Primers 5'-3' |
Promoter | F-P | CTAATCAGGTCACCACTGGATCAGAGCC | SF-P1, -518 | ACTGGATCAGAGCC |
-533, 16 | R-P | AACAACGAGGCAGCATCTTC | SF-P2, -283 | GGAGTCCTGAGGTGA |
549 bp | SR-P1, 16 | AACAACGAGGCAGCATCTTC | Â | Â |
EXON 1–3 | F-1 | CTACCTCTACCCGATCTGGTCACCA | SF-131, -64 | GATCTGGTCACCATACGCC |
-76, 1446 | R-1 | CCTTATGTACTATGACATGATAGAAGGG | SF-132, 231 | CCTGGTACTGTGTATCTTCCCACCTC |
1522 bp | Â | Â | SF-133, 788 | CCTGGTTGTAACCATGGCA |
| Â | Â | Â | SR-131, 557 | CACATGCGTTCCAAGATTCCATCTACC |
| Â | Â | Â | SR-132, 1446 | CCCCTACCTATTCCCTCCCCATTC |
EXON 4–5, 3'UTR | F-4 | CAATCCCGGCACCTC | SF-451, 2978 | CTGGCAGATCACTCGTGGTTAG |
2951, 4841 | R-4 | GAATGCCCTCTTCTA | SF-452, 3216 | GAGGGAGAGGGAGCGGCCAAACA |
1890 bp | Â | Â | SF-453, 3831 | GAATAACCATTCATAAGTGA |
| Â | Â | Â | SF-454, 3425 | GCTGAGGCAGCTGGATAACT |
(PCR fragment includes 487 bp of the 3'UTR) | SR-451, 3443 | GTTATCCAGCTGCCTCAGCGCG | ||
| Â | Â | Â | SR-452, 3966 | CCTCACAGAATTTAAGA |
| Â | Â | Â | SR-453, 4399 | ATGTGTCAAGGCTTCCAGCCCCTC |
| Â | Â | Â | SR-454, 4841 | GAATGCCCTCTTCTA |