Table 1 Oligonucleotides and restriction enzymes used for SSCP analyses of the exonic and 593 bp promoter regions of the RXRB gene.
From: On the Wegener granulomatosis associated region on chromosome 6p21.3
Exon | Oligonucleotide sense/antisense | PCR product (bp) | Restriction enzyme; length of fragments (bp) |
|---|---|---|---|
01a | CGGTATCCCTACTCTCAGCCA/GCGGGATCCAGCCAGG | 164 | - |
01b | GGTGCGAAAAGAAATGCA/CACTGGCTCGCCTGCC | 202 | - |
02 | CTCTTGTGTGTCTGTGTGCCT/GGTTAGAGGATTGGAAGGTCA | 403 | AvaII 176/227 |
03 | GACTTCCCTATTTCCCCCAT/TCTCGGAGAAGAGGAGGCT | 207 | - |
04 | TGAAGGTGTCTCCATGCAAC/AGAAGGGCATGTGGTCTAAGA | 275 | BsmFI 126/149 |
05 | CTGATGAGGCCGTAAGGATA/GTGGATTGACCCCAACACT | 280 | Eco24I 130/150 |
06 | TCCATAACTCTTACCCCCGT/CTGGGTACGCAAGGTAAGG | 255 | MboII 102/153 |
07 | CCTGATTTCTGGCTCCTGAC/CCTCTATCTACATGCCAGCCT | 209 | - |
08 | GCTGAGCTGTGACCTTTGAGT/TCTCGGAGAAGAGGAGGCT | 199 | - |
09 | CTCCTCTCTTCCCTGCCAT/CCTCATAGCACTCCCCACC | 199 | - |
10 | CTCAATCCCCTTCTCCCAC/AGTGTGAGAAGCACCACGTC | 209 | - |
Promoter (593 bp) | Â | Â | Â |
Sys1 | GGGCTTAATTCGACCCAA/GCATTTCTTTTCGCACC | 213 | - |
Sys2 | GCTAACAGGCCGGAGGAGA/GGATTGATCGGAGGATTAGCT | 212 | - |
Sys3 | CTCTCCTTTCCCGGTTTG/TCGTCTAGTTGGAAACCGAG | 234 | - |
Sys4 | CTCTTTATCCCGAACCACCT/TCTCGCGGGATCTAAAGG | 221 | - |
Sys5 | CATCACGCTGACCAGAGG/TGGTGAAAGATTAGTGTCCCA | 214 | - |