The frequent BRCA1 mutation 1135insA has multiple origins: a haplotype study in different populations

BMC Medical Genetics

Table 2 Primers used to generate the haplotype

Marker	Chromosome 17 position	Forward Primer	Reverse Primer	T_m (°C)	Other
D17S855	38,458,470	GGATGGCCTTTTAGAAAGTGG	ACACAGACTTGTCCTACTGCC	57	5 % DMSO†
D17S1322	38,465,161	CTAGCCTGGGCAACAAACGA	GCAGGAAGCAGGAATGGAAC	60	--
D17S1323	38,491,793	TAGGAGATGGATTATTGGTG	AAGCAACTTTGCAATGAGTG	60	--
D17S1325	38,891,165	AAAGGTGGCAATTCACAGTTG	GTGATAAAACTCAGTGGTACTC	65-55*	1× Q solution

All reactions were amplified for 35 cycles [30s@95°C, 30s@T_m, 30s@72°C] and contained 200 uM dCTP/dGTP/dTTP, 24 uM dATP, 10 uCi α³⁵S-dATP, 1× PCR buffer and 0.5 U of HotStar Taq DNA polymerase (QIAGEN). †Dimethyl Sulfoxide. *T_m starting at 65°C and decreasing by 1°C per cycle for 10 cycles, followed by 25 cycles at 55°C; Q solution from QIAGEN.

ISSN: 1471-2350