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Table 1 Primers used for real-time RT-PCR.

From: The host response to the probiotic Escherichia coli strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1

Product

5' Sequence 3'

Sense/antisense

Product length, bp

Tm, °C

DUSP5

AGC CCC AAG AGC AAC TGT GAT T

Sense

163

54.3

 

AGT CCC GAG AAC CTA CCC TGA G

Antisense

  

ELF3

GTC AGC AAG CCA GCC CCT ACC AC

Sense

202

62.1

 

GGA TCC CCC TTC TTG CAG TCA CGA

Antisense

  

EMP3

GCG AGA ATG GCT GGC TGA AG

Sense

204

58.3

 

GGA TCT CCT CGG CGT GAA TG

Antisense

  

MCP-1

GTC TCT GCC GCC CTT CTG TG

Sense

94

55.9

 

AGG TGA CTG GGG CAT TGA TTG

Antisense

  

MIP-2α

TTT TAG GTC AAA CCC AAG TTA GTT

Sense

150

49.0

 

TTC TTG GAT TCC TCA GCC TCT ATC

Antisense

  

MIP-2β

AAG AAG CTT ATC AGC GTA TCA T

Sense

150

50.6

 

AAT AAG TAG AAC CCT CGT AAG AAA

Antisense

  

NFκBIA

CGC CCA AGC ACC CGG ATA CAG C

Sense

193

58.7

 

TTC AGC CCC TTT GCA CTC ATA ACG

Antisense

  

RPS-9

CGC AGG CGC AGA CGG TGG AAG C

Sense

92

61.1

 

CGA AGG GTC TCC GCG GGG TCA CAT

Antisense

  

TNFαIP3

ATT GGC CTC TTT GAT ACA CTT TTG

Sense

201

52.9

 

CTC ATC CCT GCT CCT TCC CTA TCT

Antisense

  

VEGF

AAG GAG GAG GGC AGA ATC ATC ACG

Sense

192

58.8

 

CAC ACT CCA GGC CCT CGT CAT TG

Antisense

  
  1. DUSP5 indicates dual specificity phosphatase 5; ELF3, E74-like factor 3 (ets domain transcription factor, epithelial-specific); EMP3, epithelial membrane protein 3; NFκBIA, nuclear factor of kappa light polypeptide gene enhancer in B cell inhibitor, alpha; MCP-1, chemoattractant protein-1 ligand 2; MIP-2α, macrophage inflammatory protein-2 alpha; MIP-2β, macrophage inflammatory protein-2 beta; RPS-9, ribosomal protein S9; TNFαIP3, tumor necrosis factor, alpha-induced protein 3; VEGF, vascular growth factor and Tm, melting temperature.