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Table 1 Primers used for real-time RT-PCR.

From: The host response to the probiotic Escherichia coli strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1

Product 5' Sequence 3' Sense/antisense Product length, bp Tm, °C
DUSP5 AGC CCC AAG AGC AAC TGT GAT T Sense 163 54.3
  AGT CCC GAG AAC CTA CCC TGA G Antisense   
ELF3 GTC AGC AAG CCA GCC CCT ACC AC Sense 202 62.1
  GGA TCC CCC TTC TTG CAG TCA CGA Antisense   
EMP3 GCG AGA ATG GCT GGC TGA AG Sense 204 58.3
  GGA TCT CCT CGG CGT GAA TG Antisense   
MCP-1 GTC TCT GCC GCC CTT CTG TG Sense 94 55.9
  AGG TGA CTG GGG CAT TGA TTG Antisense   
MIP-2α TTT TAG GTC AAA CCC AAG TTA GTT Sense 150 49.0
  TTC TTG GAT TCC TCA GCC TCT ATC Antisense   
MIP-2β AAG AAG CTT ATC AGC GTA TCA T Sense 150 50.6
  AAT AAG TAG AAC CCT CGT AAG AAA Antisense   
NFκBIA CGC CCA AGC ACC CGG ATA CAG C Sense 193 58.7
  TTC AGC CCC TTT GCA CTC ATA ACG Antisense   
RPS-9 CGC AGG CGC AGA CGG TGG AAG C Sense 92 61.1
  CGA AGG GTC TCC GCG GGG TCA CAT Antisense   
TNFαIP3 ATT GGC CTC TTT GAT ACA CTT TTG Sense 201 52.9
  CTC ATC CCT GCT CCT TCC CTA TCT Antisense   
VEGF AAG GAG GAG GGC AGA ATC ATC ACG Sense 192 58.8
  CAC ACT CCA GGC CCT CGT CAT TG Antisense   
  1. DUSP5 indicates dual specificity phosphatase 5; ELF3, E74-like factor 3 (ets domain transcription factor, epithelial-specific); EMP3, epithelial membrane protein 3; NFκBIA, nuclear factor of kappa light polypeptide gene enhancer in B cell inhibitor, alpha; MCP-1, chemoattractant protein-1 ligand 2; MIP-2α, macrophage inflammatory protein-2 alpha; MIP-2β, macrophage inflammatory protein-2 beta; RPS-9, ribosomal protein S9; TNFαIP3, tumor necrosis factor, alpha-induced protein 3; VEGF, vascular growth factor and Tm, melting temperature.