Figure 6From: The host response to the probiotic Escherichia coli strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1 MCP-1 gene expression in small intestine. After smooth removal of the mucus layer, 3–5 tissue pieces from small intestine were cocultured with E. coli MG1655 (+ K12) or EcN (+ EcN) for 6 hours. Relative mRNA amounts were normalized with respect to expression levels of tissue pieces cultured in IMDM + 10% fetal calf serum without antibiotics (fold change = 1). The figure is representative of three independent experiments.Back to article page