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Figure 4 | BMC Medical Genetics

Figure 4

From: The host response to the probiotic Escherichia coli strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1

Figure 4

Gene expression of MCP-1 and MIP-2α in Lovo cells. Validation of mRNA expression levels of Lovo cells after 6 hours coculture with E. coli MG1655 (+ K12) or EcN (+ EcN). Relative mRNA expression levels for MCP-1, MIP-2α and RPS-9 (as internal control) were analyzed in duplicate real-time RT-PCR assays. Relative mRNA amounts were normalized with respect to expression levels of untreated Lovo cells (fold change = 1). The figure is representative of three independent experiments. MCP-1 indicates chemoattractant protein-1 ligand 2; MIP-2α, macrophage inflammatory protein-2 alpha.

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