Insulin Promoter Factor 1 variation is associated with type 2 diabetes in African Americans

BMC Medical Genetics

Table 1 Primer sequences for IPF1 SNPs

SNP NAME	FORWARD PRIMER 5' to 3'	REVERSE PRIMER 5' to 3'	SEQUENCE PRIMER 5' to 3'	Anneal Temp.
SNP1	*ATTGCTTAGCCCTAGGAATAT	AGAGGGGCCAGGGAAACCCAG	GGATTGGAGAGAGGAAA	55°C
SNP2	GACGCCAGCTGCCCGTTCA	*CTGGCTGGCCGCACTAAGAG	AATTGGAACAAAAGCAG	55°C
SNP3 rs2293942	GGCAAGGACCTCCAGTATCAG	*CCCGAGCCATTTAACAG	CCTCCAGTATCAGCGAGGAC	55°C
SNP4 rs2293943	*GGCAAGGACCTCCAGTATCAG	CCCGAGCCATTTAACAG	TGAAAAAGTCGTTTATTAGC	55°C
SNP5 rs4002827	GATATCATGGAAAATGCAGCG	*GCTTCCCAATACAGCGAGG	GCAGAAGAGAGTGAGTGTT	55°C
SNP6	GTTTCGAGAAACGTCCTCATTT	*GCTTCTGGGGTCCTGACT	CAGTCAGAGGCTGGTCA	55°C
SNP8 rs4430606	Acttcccgcgcttcgtta	*CCAGCCCCTTCCTCTTTACT	CCAGGTAGGTGCAGAAAG	52°C
SNP11	GTCGTGCGGAGCTGTCAAAGCGAG	*CTGGAGCCGGGGATTT	AGCTGTCAAAGCGAGCAGGG	55°C
InsCCG243	CACGACGTTGTAAAACGACGAGACACATCAAGATCTGGTTCCAA	GGATAACAATTTCACACAGGGCAGCGGGCGGCACA

* Denotes additional of universal primer sequence to the primer. Universal primer sequence is as follows: TCTGCTGCTCCGGTTCATAGATT-3'

ISSN: 1471-2350