Tm analysis with SYBR® Green I of 101-bp PCR products to discriminate the H/R alleles. Panel A: Melting profile without DMSO. Panel B: Melting profile with DMSO 5%. Panel C: Melting profile with DMSO 10%. Heterozygotes are depicted as thick lines, the heterozygous pattern is distinguishable from the two homozygous curves because of the shoulder, most likely due to the presence of heteroduplexes.