Table 1 Primer pairs used for mutation analysis, covering the coding region of PITX2.

2

PITX2-exon2for:

PITX2-exon2rev:

tag tct cat ctg agc cct gc

gcg att tgg ttc tga ttt cct

60

Ref: [25] this paper

3

PITX2-exon3bfor:

PITX2-exon3brev:

ttg ctc ttt gtc cct ctt tct cct

cgg agt gtc taa gtt caa gca gca

60

this paper

4a

PITX2-exon4afor:

PITX2-exon4arev:

ccg cct ctg gtt tta aga tg

gca aag acc ccc ttc ttc tc

60

this paper

4b

PITX2-exon4bfor:

PITX2-exon4brev:

ctt gac act tct ctg tca gg

aag cgg gaa tgt ctg cag g

60/56/52*

Ref: [25]

5

PITX2-exon5for:

PITX2-exon5rev:

cag ctc ttc cac ggc ttc t

ttc tct cct ggt cta ctt gg

60

Ref: [25]

6

PITX2-exon6for:

PITX2-exon6rev:

gta atc tgc act gtg gca tc

agt ctt tca agg gcg gag tt

65

Ref: [25]