Table 1 Primer pairs used for mutation analysis, covering the coding region of PITX2.
| exon | primer name | sequence 5'> 3' | TA°C | reference |
|---|---|---|---|---|
| 2 |
PITX2-exon2for: PITX2-exon2rev: |
tag tct cat ctg agc cct gc gcg att tgg ttc tga ttt cct | 60 | Ref: [25] this paper |
| 3 |
PITX2-exon3bfor: PITX2-exon3brev: |
ttg ctc ttt gtc cct ctt tct cct cgg agt gtc taa gtt caa gca gca | 60 | this paper |
| 4a |
PITX2-exon4afor: PITX2-exon4arev: |
ccg cct ctg gtt tta aga tg gca aag acc ccc ttc ttc tc | 60 | this paper |
| 4b |
PITX2-exon4bfor: PITX2-exon4brev: |
ctt gac act tct ctg tca gg aag cgg gaa tgt ctg cag g | 60/56/52* | Ref: [25] |
| 5 |
PITX2-exon5for: PITX2-exon5rev: |
cag ctc ttc cac ggc ttc t ttc tct cct ggt cta ctt gg | 60 | Ref: [25] |
| 6 |
PITX2-exon6for: PITX2-exon6rev: |
gta atc tgc act gtg gca tc agt ctt tca agg gcg gag tt | 65 | Ref: [25] |