Table 1 Primer sequence, PCR and Pyrosequencing conditions for CYP3A4 and CYP3A5 SNP analysis.
SNP | Forward Primera(5'-3') | Reverse Primera(5'-3') | Number of Cycles | Annealing Temperature (° C) | Internal Primerbc(5'-3') | Sequence to Analyzed |
|---|---|---|---|---|---|---|
CYP3A4*1B | aggacagcccatagagacaagg | *atcaatgttactggggagtcc | 55 | 55 | F-ccatagagacaagggca | A/GGAGA |
CYP3A4*2 | aacaatccacaagacccctt | *atcttcaaatgtactacaaatcactga | 55 | 55 | F-tttggatccattctttc | TCTC/TAAT |
CYP3A4*3 | cgtggaaccagattcagcaa | *gaaggagaagttctgaaggactctg | 55 | 65 | F-ccagaaactgcattgg | CAT/CGAGG |
CYP3A5*3C | *cccacgtatgtaccacccagc | attagggtgtgacacagcaaga | 55 | 65 | R-ccaaacagggaagaga | TAC/TTGe |
CYP3A5*6 | *tctttggggcctacagcatg | aaagaaataatagcccacatacttattgagag | 55 | 62 | R-agaaaccaaattttaggaa | CTTC/TTTAGe |