TM4SF10 gene sequencing in XLMR patients identifies common polymorphisms but no disease-associated mutation

BMC Medical Genetics

Table 2 PCR conditions and primer sequences used to amplify TM4SF10 gene fragments from total genomic DNA

Amplicon	Size	Primer sequence	PCR conditions
Ex1	442 bp	fw: AGAGCCCCGAGGGAGCGA, rev: GGGGACAGGCGGTGACTG	T_anneal = 55°C, 10% DMSO
Ex2	447 bp	fw: AAATCCTAGCAAACCCCTGG, rev: TCTGCATAGGAAAGGAAGATGG	T_anneal = 50°C
Ex3	447 bp	fw: CCATCTAGAACAAGCCATCTTTAA, rev: TAAATCAACTGAGCAAACTGCTTG	T_anneal = 50°C
3'UTR F1	959 bp	fw: GGCCTGGGGTGCAACTATAT, rev: TAGGCAAATGTATGTGGAGGGT	T_anneal = 55°C, 10% DMSO
3'UTR F2	1101 bp	fw: ATTGGTGCCTCAGCCCTATCTA, rev: GCAACCATTCTTAAGACAAGCT	T_anneal = 57°C, 20% Q solution
3'UTR F3	1130 bp	fw : CAGTATGTTCTGGTTTTGGCCC, rev : TATCTAACAATGGGTTTGTGGC	T_anneal = 57°C, 20% Q solution
3'UTR F4	1097 bp	fw : CCTTCTCAGCAAAGAGCCCTAC, rev : AAGGATCTTGGGAGATAATTTG	T_anneal = 57°C, 20% Q solution

ISSN: 1471-2350