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Table 2 MRF-SSCP for mutation analysis of the full-length PKD1 cDNA

From: Novel and de novo PKD1 mutations identified by multiple restriction fragment-single strand conformation polymorphism (MRF-SSCP)

PCR Fragment
from PKD1-cDNA
Size (bp) before
endonuclease digestion
Restriction
endonuclease used
Restriction fragment length
(bp) in MRF-SSCP (5' > 3')
SI 1 1,554 Pvu II+Xcm I+Ava II 209, 318, 99, 249, 294, 385
   Afl III+Sac I+Ava II 209, 282, 384, 238, 312, 129
SI 2 1,678 Pvu II+Hinf I 429, 93, 366, 277, 295, 218
   Sma I+Bgl I 108, 245, 292, 605, 123, 306
SI 3 1,575 Bgl I+Hinf I 233, 81, 424, 198, 325, 202, 112
   Taq I 429, 215, 187, 243, 501
SI 4 1,473 Bgl I+Hha I 131, 159, 96, 380, 108, 476, 123
   Ava II 178, 252, 475, 204, 253, 111
SI 5 1,663 Sau3A I+Bgl I 127, 387, 377, 188, 226, 169, 189
   Ava II+Bgl I 116, 324, 423, 216, 141, 85, 358
SI 6 1,506 Ava II+Bgl I 173, 307, 294, 402, 187, 143
   BstU I+BstE II 313, 344, 265, 190, 70, 239, 85
SI 7 1,518 BstU I+Hinf I 112, 287, 424, 389, 201, 105
   Ava II+Hinf I 197, 202, 398, 315, 192, 109, 105
SI 8 1,539 Pvu II+Pst I+BstE II 337, 199, 233, 505, 218, 185
   Taq I+Bgl I 190, 141, 222, 344, 474, 98, 208
SI 9 1,650 Pvu II 190, 351, 81, 180, 453, 135, 12, 248
   Taq I+Bgl I 288, 318, 197, 529, 84, 234