Primer | Primer Sequence (5'->3') | Nucleotide Position | Location in PKD1Transcript | PCR Product Size (bp) | Annealing Temperature (°C) | Mg2+ Conc. (mM) |
---|---|---|---|---|---|---|
 | cDNA amplification |  |  |  |  |  |
TH1F | CTGGGGACGGCGGGGCCATGCG | 175–196 a | 5' UTR | 13,634 | 68 | 1.2 |
TH1B | GGCCTGGGGCAAGGGAGGATGACAA | 13808–13784 a | 3' UTR |  |  |  |
SI1F | ACGGCGGGGCCATGCGCGCGCTGCCCTAAC | 181–210a | 5'UTR | 1,554 | 72 | 3.0 |
SI1B | CAGTGCTCGGCTGTGGCTGGGTGTGGCTCC | 1734–1705a | Exon 7 |  |  |  |
SI2F | AGGAGCCTAGACGTGTGGATCG | 1595–1616a | Exon 6–7 | 1,678 | 65 | 1.5 |
SI2B | CCTGCATCCTGTTCATCCGCTC | 3272–3251a | Exon 13 |  |  |  |
SI3F | CATCAACGACAAGCAGTCCCT | 3115–3135a | Exon 12 | 1,575 | 60 | 1.5 |
SI3B | CCACGGCCCACAGCAGAGAA | 4689–4670a | Exon 15 |  |  |  |
SI4F | ATCTCTGCTGCCAATGACTCAG | 4562–4583a | Exon 15 | 1,473 | 60 | 1.5 |
SI4B | GGGGAAGCTGTGGGAGAAAC | 6034–6015a | Exon 15 |  |  |  |
SI5F | TCAATGCCTCCAACGCAGTCAGC | 5808–5830a | Exon 15 | 1,663 | 65 | 1.5 |
SI5B | GTCTCATCCAGCACCAGCGTCTTG | 7470–7447a | Exon 18 |  |  |  |
SI6F | AGCAGCGGCTCCAAGCGAG | 7400–7418a | Exon 17 | 1,506 | 65 | 3.0 |
SI6R | CACAACGGAGTTGGCGGAGT | 8905–8886a | Exon 23 |  |  |  |
SI7F | CCTTTCCCTTTGGCTATATCAG | 8709–8730a | Exon 23 | 1,518 | 60 | 1.5 |
SI7B | CCAGGTAGACGGGATAGACAAC | 10226–10205a | Exon 30 |  |  |  |
SI8F | GCCACCTGCTGCGTTCTCCT | 10052–10071a | Exon 29 | 1,539 | 65 | 1.5 |
SI8B | CGTCCCCGAGCCATTGTGAG | 11590–11571a | Exon 40 |  |  |  |
SI9F | CTTCAGCACCAGCGATTACGACGTT | 11533–11557a | Exon 40 | 1,650 | 65 | 1.5 |
SI9B | AGAAAGTAATACTGAGCGGTGTCCACTC | 13182–13155a | 3' UTR |  |  |  |
 | Genomic DNA amplification |  |  |  |  |  |
SI3.1F | GCAACGTCACCGTGAACTACAACGTAACCG | 26340–26369 b | Exon 13 | 18,099 | 61 | 1.0 |
Ex34B | GAGCAGGTCCGTTTCCATGTGGGTGTCTTG | 44438–44409 b | Exon 34 |  |  |  |
SI4.2F | CTTCCCCACCAACCACACGGTACAGC | 29064–29039 | Exon 15 | 486 | 62 | 1.0 |
SI4.2B | AGGCCACTCACAGGCACCTGCACATC' | 29523–29498 | Exon 15 |  |  |  |
SI4.1F | AGCCAACGCCACCGTGGAA | 29476–29494b | Exon 15 | 372 | 60 | 1.5 |
SI4.1B | GCAGCCAGCAGGATCTGAAAATG | 29847–29825 b | Exon 15 |  |  |  |
SI6.1F | CGCTGTGCACGCCCTCACCACCAAGGT | 32835–32861 b | Exon 18 | 480 | 60 | 1.5 |
SI6.1B | GTGCAGCCAGACTGTGAGCCCCGTTGC | 33314–33288 b | Exon 20 |  |  |  |
SI7.2F | GGAGTTACCATCTGAACCTCTCCAG | 38978–39002 b | Exon 25 | 698 | 60 | 1.0 |
SI7.2B | CAGGATGAACACACGAGCCCTTCACAC | 39675–39649 b | IVS 26 |  |  |  |
SI8L | TTCTTTGACAAGCACATCTGGC | 41600–41579 | Exon 29 | 336 | 57 | 1.0 |
SI7B | CCAGGTAGACGGGATAGACAAC | 41915–41894 | Exon 30 |  |  |  |