Table 1 PCR conditions and primers for analyzing TP73 SNPs
From: TP73allelic expression in human brain and allele frequencies in Alzheimer's disease
Analysis | SNP Location | PCR Primers (Forward; Reverse) | Anneal. Temp. | Size (bp) | Restriction Enzyme |
|---|---|---|---|---|---|
Promoter construct | P3 promoter | (F) GCACCACTGCAGAGCCCCTCCC (R) AACAACAAAACCCGCGGCCCACC | 60°C | 675 | N/A |
Genotyping | P3 promoter | (F) AGAGCCCCTCCCTGGCTGTGCC (R) CGAGGATGCTGGGGAACATGC | 65°C | 748 | SpeI |
Genotyping | Exon 5 | (F) ACAGGGGTGCAGTTGGGAC (R) CTGGGAAAATGGGGGCTGGTG | 60°C | 403 | BanI |
Allelic Expression | Exon 4–6 RT-PCR | (F) CTTTCCAGCAGTCCAGCACGG (R) CGTGGTTGGGGCAGCGTTTCA | 60°C | 203 | BanI |
Control for Allelic Expression | Exon 5 Genomic PCR | (F) TTGGGACCACTGGTCTCA (R) GTGGTTGGGGCAGCGTTTCA | 60°C | 235 | BanI |